To form organoids, stem cells are transplanted into the extracellular matrix. Organoids can help pave the road for precision medicine through genetic editing, pharmaceutical development, and cell therapy. Studies on the use of stem-cell-derived organoids in regenerative medicine, organoids as 3D culture models for EVs analysis, and organoids for precision medicine were included. Results: According to the included studies, organoids have various origins, types, and applications in regenerative and precision medicine, as well as an important role in studying extracellular vesicles.

Results: The results implied that six hub-bottlenecks including ACTB, GAPDH, HSP90AA1, HSPA8, HSP90AB1, and HSPA5 were present in the PPI network; however, only the last central protein was among DEPs. ClueGO CluePedia identified five related biological processes and three action types of their connections. Conclusion: The proposed crucial proteins and their linked biological processes may represent as key players in the anticancer underlying mechanism of red propolis.

Over the last few years, protein-based nanofibrous biomaterials have been used for better controlling dementia. PubMed, Scopus, and ISI Web of Science were consulted for available articles on nano-based fibrous systems for the treatment and diagnosis of dementia (up to October 2022). Eleven studies evaluated nanofibrous electrospun biomaterials for better dementia control. Additionally, four studies inspected stem cell (SC) transplantation on nano-based fibrous scaffolds for better treatment of dementia. Finally, two from the final four studies considered nano-based fibrous systems for the enhanced treatment of dementia.

Many patients with type 1 diabetes fast during Ramadan according to (or against) medical and jurisprudence advice. The current scoping review protocol aims at systematic analysis and mapping of existing literature in the field and highlighting scientific gaps. Considering the fact that Ramadan Fasting is a cultural-dependent subject, which may be studied in the Middle Eastern and Islamic Countries in languages other than English, local Persian and Arabic Databases will also be included.

Assessment of molecular mechanism of yellow onion extract on cell line Caco-2 was the aim of this study. The gene expression profiles of Caco-2 cells in the presence of yellow onion extract versus control cells were analyzed via GOE2R software. The significant differentially expressed genes (DEGs) were assessed via network analysis and the central nodes were enriched via gene ontology. Results: Thirteen central nodes including JUN, ATF3, DUSP1 VEGF, CDKN1A, SNAI1, HSPB1, MCL1, SQSTM1, SREBF1, MAP1LC3B, EZR, and DUSP5 were identified.

Abstract Background and objectives: Radix Scropholaria is dried root Scrophularia ningpoensis Hemsl. which is used uses as a drug against several diseases. In the present study, the crucial affected proteins of rat liver in the presence of radix Scrophularia have been investigated. Methods: The differentially expressed proteins (DEPs) were downloaded from literature. The significant DEPs plus 100 added first neighbors were determined and included in

Infusion of allogeneic NK cells might be a complementary treatment to boost immune system function in COVID-19 patients. In this project, we focused on COVID-19 patients with low inspiratory capacity (LIC). This project aims to evaluate the feasibility and safety of allogeneic NK cell infusion as an intervention for respiratory viral disease. Methods: A non-blind two arms pilot study was designed and conducted after signing the consent form. Ten matched patients, in terms of vital signs and clinical features, were enrolled in the control and intervention groups.

Therefore, in our study, the impact of three-dimensional (3D) co-culture of human fetal pancreatic-derived mesenchymal cells (hFP-MCs) with human embryonic stem cell-derived pancreatic progenitors (hESC-PPs) development towards endocrine and beta cells was assessed. However, they showed higher expression of DESMIN compared to BM-MSCs. After co-culture of hESC-PPs with hFP-MCs, the pancreatic progenitor (PP) spheroids generated in Matrigel had higher expression of NGN3 and INSULIN than BM-MSCs co-culture group, which shows an inductive impact of pancreatic mesenchyme on hESC-PPs beta-cells maturation.

To manufacture clinical-grade MSCs, animal-free culture protocols are preferred. A side-by-side comparison was made to evaluate the effect of each serum on proliferation rate, cell cycle, senescence, multi-lineage differentiation capacity, immunophenotype, and tumorigenesis of FPMSCs. Results: Flow cytometry analysis and three-lineage differentiation ability demonstrated that fibroblast-like cells obtained from primary culture had MSCs' characteristics. In conclusion, HS can effectively replace FBS for clinical-grade FPMSCs manufacturing.