Abstract Background and objectives: Radix Scropholaria is dried root Scrophularia ningpoensis Hemsl. which is used uses as a drug against several diseases. In the present study, the crucial affected proteins of rat liver in the presence of radix Scrophularia have been investigated. Methods: The differentially expressed proteins (DEPs) were downloaded from literature. The significant DEPs plus 100 added first neighbors were determined and included in

Infusion of allogeneic NK cells might be a complementary treatment to boost immune system function in COVID-19 patients. In this project, we focused on COVID-19 patients with low inspiratory capacity (LIC). This project aims to evaluate the feasibility and safety of allogeneic NK cell infusion as an intervention for respiratory viral disease. Methods: A non-blind two arms pilot study was designed and conducted after signing the consent form. Ten matched patients, in terms of vital signs and clinical features, were enrolled in the control and intervention groups.

Therefore, in our study, the impact of three-dimensional (3D) co-culture of human fetal pancreatic-derived mesenchymal cells (hFP-MCs) with human embryonic stem cell-derived pancreatic progenitors (hESC-PPs) development towards endocrine and beta cells was assessed. However, they showed higher expression of DESMIN compared to BM-MSCs. After co-culture of hESC-PPs with hFP-MCs, the pancreatic progenitor (PP) spheroids generated in Matrigel had higher expression of NGN3 and INSULIN than BM-MSCs co-culture group, which shows an inductive impact of pancreatic mesenchyme on hESC-PPs beta-cells maturation.

To manufacture clinical-grade MSCs, animal-free culture protocols are preferred. A side-by-side comparison was made to evaluate the effect of each serum on proliferation rate, cell cycle, senescence, multi-lineage differentiation capacity, immunophenotype, and tumorigenesis of FPMSCs. Results: Flow cytometry analysis and three-lineage differentiation ability demonstrated that fibroblast-like cells obtained from primary culture had MSCs' characteristics. In conclusion, HS can effectively replace FBS for clinical-grade FPMSCs manufacturing.

Acute respiratory distress syndrome (ARDS) is one of the most important causes of death in COVID-19 patients. Mesenchymal stem cells have been the subject of many clinical trials for the treatment of ARDS because of their immunomodulatory, anti-inflammatory, and regenerative potentials. The aim of this phase I clinical trial was the safety assessment of allogeneic placenta-derived mesenchymal stem cells (PL-MSCs) intravenous injection in patients with ARDS induced by COVID-19. Methods: We enrolled 20 patients suffering from ARDS caused by COVID-19 who had been admitted to the intensive care unit.

efficacy of an injectable tissue-engineered construct composed of rat bone marrow mesenchymal stem cells (rBMMSCs), platelet-rich plasma (PRP), and collagen type I in rat model of OA. construct was formed by a combination of appropriate amount of collagen type I, PRP and rBMMSCs. In vitro studies were tissue-engineered construct was injected in knee joints of rat models of OA (24 rats in 4 groups: OA, OA MSC, OA collagen of knee joint samples were performed to evaluate the effect of each treatment on OA. Tissue-engineered construct was

This virus can affect all systems of the body and its symptoms vary from a simple upper respiratory infection bacterial co-infection is another problem in COVID-19 which affects the prognosis of patients. pandemic, various approaches and therapeutics were introduced for treating COVID-19 patients. are not helpful enough, especially for complicated cases. Therefore, the therapeutic benefits of mesenchymal stem cells, especially their antimicrobial activity, will help us understand how to treat COVID-19. Herein, mesenchymal stem cells may stop the immune system from becoming overactive in COVID-19 patients.

This meta-analysis revealed that the PBMT utilizes beneficial anti-inflammatory effects and modulation of the immune system on lung damage in animal models and clinical studies. However, animal models and clinical studies appear limited considering the evidence’s .quality; therefore, large clinical trials are still required